Sunrise Course:
Mobile Lipids in Disease
Organizers: Carles Arús, Ph.D. and Edward J. Delikatny, Ph.D.
Skill Level: Advanced
Tuesday, 21 April 2009 - Friday, 24 April 2009
07:00 - 08:00

This course of four one hour sessions will consist of 2 or 3 lectures each morning followed by a discussion. Most speakers will be from the NMR field with expertise in detection and interpretation of the NMR visible mobile lipid (ML) region of the 1H NMR spectrum, in humans and in cell and animal models of human disease. Additional speakers will be drawn from the field of lipid metabolism and lipid droplet dynamics. The topics will range from the biochemical and subcellular origin of ML, the dynamics of ML inside cells, their NMR measurable biophysical parameters, in model systems, cells, biopsies and in vivo, and finally the correlation between their MRS observable changes in vivo and disease detection and diagnosis (cancer, diabetes, liver disease).
Upon completion of this case based session, participants should be able to:
  • Recognize the various ML resonances detectable in the 1H NMR spectral pattern of a tissue or organ;
  • Select optimal acquisition conditions to detect ML resonances and appreciate their possible variation in biological samples;
  • Describe the possible biochemical origins of ML;
  • List possible causes for qualitative and quantitative changes in the ML pattern;
  • Interpret qualitative and quantitative changes in the ML pattern of cells and tissues; and
  • List possible clinical applications of in vivo ML detection for disease diagnosis, staging and response to therapy.

Tuesday, 21 April 2009
  Mobile Lipids (ML) from the Cellular Point of View  
07:00 MRS Resonances as Indicators of Altered Phospholipid and
Neutral Lipid Metabolism in Living Cells
Franca Podo, D.Sc.
07:20 Mobile Lipids and Apoptosis Kevin M. Brindle, D.Phil.
07:40 Dynamics of NMR Visible Mobile Lipids in Cultured Cells Carles Arús, Ph.D.
08:00 Adjournment  
Wednesday, 22 April 2009  
  ML Compartment Studies in Model Systems, Cells and in vivo by NMR and Other Methods
07:00 Mobile Lipid Accumulation as Stress Response Edward J. Delikatny, Ph.D.
07:20 ML in Model Systems and Plasma/Serum: Micelles, Lipoproteins Alkystis Phinikaridou, M.S.
07:40 Relaxation and Diffusion Measurements in vitro, ex vivo and in vivo Chantal Rémy, Ph.D.
08:00 Adjournment  
Thursday, 23 April 2009  
  Cancer, Brain, and Other  
07:00 Animal Studies Risto A. Kauppinen, M.D., Ph.D.
07:30 Clinical Studies Martin O. Leach, Ph.D.
08:00 Adjournment  
Friday, 24 April 2009  
  Muscle (IMCL and EMCL), Heart, Liver, Diabetes  
07:00 Muscle. IMCL, EMCL and Muscle Metabolism in vivo Chris Boesch, M.D., Ph.D.
07:20 Lipotoxicity, Diabetes, Steatosis, Steatohepatitis - Preclinical Animal Models Rheal Antoine Towner, Ph.D.
07:40 Clinical. Heart, Liver, Diabetes Linda S. Szczepaniak, Ph.D.
08:00 Adjournment