Weekend Educational Course:
Cellular and Molecular Imaging
Organizer: Patrick Winter, Ph.D.
Skill Level: Basic
Saturday, 18 April 2009
08:00 - 17:30

The first part of this one day course will focus on technological aspects of cellular and molecular imaging, covering the underlying physical principals and design criteria for three primary classes of MRI contrast agents (paramagnetic, super-paramagnetic and activatable agents). Part 2 will focus on biological labeling techniques, including in vivo targeting of MRI contrast agents to biomarkers, labeling strategies for tracking cellular migration and homing, and cell tracking using methods that produce positive image contrast. Part 3 will outline diverse applications of cellular and molecular imaging techniques, demonstrating specific contrast agent designs to meet the unique needs of each: ultra-high field imaging, nanotechnology and detection of angiogenesis. The final portion covers the use of molecular and cellular MRI for detecting and monitoring the response to therapy. In particular, three broad applications will be presented, including two of the most prevalent and debilitating diseases (cancer d cardiovascular disease) and the newly emerging field of gene therapy. Apart from the technological aspects, most of the lectures in the course will highlight recent applications of cellular and molecular imaging in biomedical research.
Upon completion of this case based session, participants should be able to:
  • Explain basic concepts and the potential of cellular and molecular imaging as a basic research tool, for diagnostics of disease processes and for monitoring therapeutic interventions, and explain the role of imaging cellular and molecular markers versus structural and physiological readouts;
  • Explain image contrast principles of the various reporter moieties (paramagnetic, superparamagnetic and activatable MRI contrast agents) and recognize the strengths and weaknesses of each;
  • Describe the techniques utilized to functionalize contrast agents for the purpose of binding or interacting with biological targets and the resulting imaging procedures to detect cellular or molecular biomarkers;
  • List design criteria for contrast agents specifically developed for various biomedical applications;
  • Assess the potential of cellular and molecular imaging concepts for combining therapeutic interventions and diagnostics.

Saturday, 18 April 2009
08:00 Introduction Patrick M. Winter, Ph.D.
08:35 Paramagnetic Agents Eva Jakab Toth, Ph.D.
09:10 Iron Oxide Particles Marlène Wiart, Ph.D.
09:45 Activatable Agents A. Dean Sherry, Ph.D.
10:20 Break - Meet the Teachers  
  Labeling Strategies  
10:40 In vivo Targeting Dmitri Artemov, Ph.D.
11:15 Cell Labeling Olivier Clément, M.D., Ph.D.
11:50 Cell Tracking with Positive Contrast Michael T. McMahon, Ph.D.
12:25 Break - Meet the Teachers  
13:30 Ultra High Field Glenn A. Walter, Ph.D.
14:05 Nanotechnology Lon J. Wilson, Ph.D.
14:40 Imaging Angiogenesis Michal Neeman, Ph.D.
15:15 Break - Meet the Teachers  
  Therapy Monitoring  
15:45 Cancer Kevin M. Brindle, D.Phil.
16:20 Cardiovascular Disease Dara L. Kraitchman, V.M.D., Ph.D.
16:55 Gene Therapy Ann-Marie Broome, Ph.D.
17:30 Adjournment - Meet the Teachers