ISMRM 23rd Annual Meeting & Exhibition • 30 May - 05 June 2015 • Toronto, Ontario, Canada

Combined Educational & Scientific Session

Musculoskeletal Functional Imaging: Mechanics & More

SKILL LEVEL: Intermediate

ORGANIZERS: Eric Y. Chang, M.D., Richard Kijowski, M.D., William B. Morrison, M.D., Ravinder R. Regatte, Ph.D. & Siegfried Trattnig, M.D.

Monday 1 June 2015

This hybrid course will provide an in-depth discussion of advanced functional imaging of musculoskeletal systems for evaluating joint mechanics, structure and composition. The lectures will discuss the technical aspects, the clinical and research applications of emerging MR methods, joint mechanics and function. The didactic lectures will be followed by presentation of scienctifc abstracts discussing novel MR methods for evaluating joint mechanics,structure and composition. This course is intended to bridge the gap between basic science research and clinical translation.

Target Audience
Radiologists and physicists who perform clinical imaging and/or research of joint mechanics and gait using advanced techniques.

Educational Objectives
Upon completion of this course, participants should be able to:
• Discuss the role of joint mechanics and gait in development of cartilage degeneration;
• Review the role of ligaments, tendons, menisci and cartilage in knee joint function; and
• Implement advanced imaging of joint mechanics and potential clinical applications.

Moderators: Matthew F. Koff, Ph.D., Bruce M. Damon, Ph.D.
16:30   Techniques: Joint Mechanics & Gait
Thor Besier, Ph.D.
17:00   Clinical Applications
Garry E. Gold, M.D.
17:30 0250.   Evaluation of the Relationship between IVIM Microvascular Blood Flow and Exercise Duration in Shoulder Muscles after Lift-off Test
Christian Federau1, Jean-Baptiste Ledoux1, Patrick Omoumi1, and Fabio Becce1
1CHUV, University Hospital Lausanne, Lausanne, Vaud, Switzerland

Intravoxel Incoherent Motion (IVIM) offers the possibility to study microvascular perfusion properties of skeletal striated muscle. This study demonstrates the selective increase in IVIM perfusion parameters in subscapularis and posterior and lateral deltoid muscles after a lift-off test, which is a clinical test recognized to be selective for those muscles. This method is a promising new tool to investigate skeletal striated muscle physiology as well as perfusion-related muscular disorders, such as peripheral arteriopathies and diabetes mellitus-related microangiopathies.

17:42 0251.   
Quantitative NMR imaging of the short-T2 components in the SKM tissue: alterations observed in myopathic patients
Ericky Caldas de A. Araujo1, Noura Azzabou1, Alexandre Vignaud2, Geneviève Guillot3, and Pierre G. Carlier1,4
1NMR Laboratory, Institute of Myology, Paris, Île-de-France, France, 2CEA/DSV/I2BM/NeuroSpin/UNIRS, Gif Sur Yvette, Île-de-France, France,3IR4M/UMR8081/CNRS, University Paris-SUD, Orsay, Île-de-France, France, 4NMR Laboratory, CEA/I2BM/MIRCen, Paris, Île-de-France, France

UTE methods offer the possibility of acquiring NMR signal from protons in the hydration layers of the collagen. We have applied the UTE method for imaging the short-T2 signal in SKM tissue, which we hypothesise to reveal intramuscular connective tissue content. Dual-echo acquisition and selective long-T2 suppression methods fail to isolate the short-T2 signals in SKM. We propose a new method for processing UTE data, resulting in signal issued exclusively from the short-T2 components in the SKM. The proposed method was applied to investigate the SKM tissue of eight healthy volunteers and three patients diagnosed with NMDs.

17:54 0252.   
In vivo diffusion MR study at 7T of hindlimb muscles in a mouse model of Duchenne muscular dystrophy - permission withheld
Paola Porcari1, Elizabeth Greally2, Volker Straub2, and Andrew M Blamire1
1Newcastle Magnetic Resonance Centre, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom, 2Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom

In this study, we investigated whether diffusion MRI might be a useful method to evaluate differences in the microarchitecture of hindlimb muscles between dystrophic (mdx) and healthy (wt) mice of three groups of age. Mice were investigated at multiple diffusion times and significant differences between the diffusion properties of hindlimb muscles in healthy and diseased mice were found for long diffusion times. High specificity and sensitivity of the method was demonstrated and also confirmed by the preliminary results of the histological assessment, thus suggesting a powerful and straightforward application on humans.

18:06 0253.   The relationship of walking speed metrics to phosphorus magnetic resonance spectroscopy (31P-MRS) bioenergetic measurements in the Baltimore Longitudinal Study of Aging (BLSA)
Seongjin Choi1, David A. Reiter2, Kenneth W. Fishbein2, Eleanor M. Simonsick1, Richard G. Spencer2, and Luigi Ferrucci3
1Translational Gerontology Branch, NIH/National Institute on Aging, Baltimore, Maryland, United States, 2Laboratory of Clinical Investigation, NIH/National Institute on Aging, Baltimore, Maryland, United States, 3Intramural Research Program, NIH/National Institute on Aging, Baltimore, Maryland, United States

Gait speed is an important predictor of morbidity in the elderly and skeletal muscle mitochondrial function plays a central role in mobility. 31P MRS of skeletal muscle permits evaluation of mitochondrial function through the phosphocreatine recovery rate (kPCr) after exercise. In this cross-sectional study using the Baltimore Longitudinal Study of Aging cohort we assessed associations between 31P MRS-determined mitochondrial function, age and four walking speed metrics. We found that kPCr, a marker of mitochondrial synthetic capacity, had the strongest correlation with 400 m walk performance at maximum pace, which was the most strenuous ambulatory exercise among the four walking metrics.

18:18 0254.   Mitochondrial NADH in vivo: functional test reveals a natural indicator of oxidative phosphorylation in 31P spectrum.
Kevin E Conley1, Amir Ali1, and Sharon Jubrias1
1Radiology, University of Washington, Seattle, WA, United States

Nicotinamide adenine dinucleotide (NAD) compartmentation underlies the role of this co-enzyme as a co-factor in oxidative phosphorylation, glycolysis and mitochondrial biogenesis. Here we identify a unique resonance for mitochondrial NADH by phosphorus magnetic resonance spectroscopy (31P MRS) in vivo. This mitochondrial NADH pool is identified by chemical standards and dynamic changes in the NAD+ and NADH peaks with activation of oxidative phosphorylation in human muscle. The end result is non-invasive detection of NADH in cell vs. mitochondria, which provides sensitive natural indicators of redox compartmentation and oxidative phosphorylation in human tissues in vivo.

18:30   Adjournment & Meet the Teachers