ISMRM 24th Annual Meeting & Exhibition • 07-13 May 2016 • Singapore

Combined Educational & Scientific Session: Imaging Metabolism: What Can We Detect & How?

Skill Level: Intermediate

Organizer: Anke Henning, Ph.D. & N. Jon Shah, Ph.D.

Thursday 12 May 2016

The course reviews metabolic processes and related metabolites, compounds and ions as well as metabolic pathways that are accessible by non-invasive or minimally invasive metabolic imaging methods.

Target Audience
Basic Scientists, Physiologists, Clinicians, MR Physicist who support this groups, PhD students and Postdoctoral fellows in related research areas.

Educational Objectives
Upon completion of this course, participants should be able to:

  • Understand which metabolic processes can be imaged;
  • Understand complementarity of metabolic imaging methods with respect to the metabolic processes that can be assessed by them; and
  • Understand differences between the metabolic imaging methods with regard to sensitivity, specificity, accuracy and precision as well as applicability to animals and humans.

Moderators: Anke Hennig, N.Jon Shah
Imaging Membrane & Protein Metabolism
Kristine Glunde
This presentation will provide an overview of current 1H and 31P magnetic resonance spectroscopy (MRS) approaches as well as chemical exchange saturation transfer (CEST) and amide proton transfer (APT) techniques that detect membrane and protein metabolism in cancer, along with a discussion of the detected molecules in the realm of cancer diagnosis and treatment monitoring.

Imaging Neurotransmission - Permission Withheld
In-Young Choi1
1University of Kansas Medical Center
Imaging Energy Metabolism
Craig R. Malloy1
1University of Texas Southwestern
Metabolic profiling of in vivo brain rodent models by relaxation-enhanced 1H MRS of the downfield region at 21.1 T
Tangi Roussel1, Jens T. Rosenberg2, Samuel Colles Grant2,3, and Lucio Frydman1,2
1Chemical Physics, Weizmann Institute of Science, Rehovot, Israel, 2Center for Interdisciplinary MR, National High Magnetic Field Laboratory, Tallahassee, FL, United States, 3Chemical & Biomedical Engineering, Florida State University, Tallahassee, FL, United States
This study explores new opportunities that ultra-high field combined with non-water-suppressed 1H MRS methodologies make possible regarding the profiling of signals that resonate downfield from the water peak. Studies were carried out on rats using a 21.1-T ultra-widebore system, and focused on quantitatively analyzing the metabolic concentration changes for ischemic stroke and glioblastoma tissues. A general decrease in the relative metabolic concentrations were observed for both pathologies, certain molecules depart from this trend: lactate, glutathione (stroke), choline and UDP-Nacetyl hexosamines (glioma). Potential explanations for these features and new research avenues opened by these types of measurements are discussed.

Study of the Mutated Isocitrate Dehydrogenase 1 in Acute Myeloid Leukemia Using Hyperpolarized [1-13C]a-ketoglutaric Acid
Eugen Kubala1,2,3, Kim A. Muñoz Álvarez1, Oliver Dovey4, Steffen J. Glaser2, Markus Schwaiger1, George S. Vassiliou4, Roland Rad5,6, Rolf F. Schulte3, and Marion I. Menzel3
1Department of Nuclear Medicine, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany, 2Department of Chemistry, Technische Universität München, Munich, Germany, 3General Electric Global Research, Munich, Germany, 4The Welcome Trust Sanger Institute, Hinxton/Cambridge, United Kingdom, 5Department of Medicine II, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany, 6Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Munich, Germany
Previous studies suggest that isocitrate dehydrogenase 1 (IDH1) mutation plays a significant role in the cancerous metabolome. Among other alternations, expression of branched chain amino-acid transaminase 1(BCAT1) is reduced, causing a decrease of α-ketoglutaric acid (αKG) to glutamic acid metabolic pathway. More importantly, the mutated IDH1 catalyzes a reaction of αKG to the oncometabolite 2-hydroxyglutarate. In this study we proved that these metabolic changes can be measured using hyperpolarized [1-13C]α-KG and 13Cmetabolic magnetic resonance spectroscopy (13CMMRS) in acute myeloid leukemia cell line in vitro.

CEST Imaging of the Serotonin Pathway
Rafal Janik1, Lynsie A.M. Thomason2, and Greg J. Stanisz1,2,3
1Medical Biophysics, University of Toronto, Toronto, ON, Canada, 2Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, 3Department of Nerurosurgery and Pediatric Neurosurgery, Medical University of Lublin, Lublin, Poland
A novel method for the detection of brain 5-HT, tryptophan, and 5-HIAA is presented. The method relies on the chemical exchange of an amide proton which is shifted outside the normal range for amide protons. This is demonstrate in-vivo in a rat model of 5-HT increase.

Towards “non-invasive histology” of the brain by diffusion-weighted MR spectroscopy in vivo: comparison between diffusion-extracted synthetic cells and real histology in the mouse and primate brain
Marco Palombo1,2, Clémence Ligneul1,2, Chloé Najac1,2, Juliette Le Douce1,2, Julien Flament1,2, Carole Escartin1,2, Philippe Hantraye1,2, Emmanuel Brouillet1,2, Gilles Bonvento1,2, and Julien Valette1,2
1CEA/DSV/I2BM/MIRCen, Fontenay-aux-Roses, France, 2CNRS Université Paris-Saclay UMR 9199, Fontenay-aux-Roses, France
A new diffusion-weighted MRS paradigm, combining advanced modeling with metabolites diffusion measurements at long diffusion times, is applied in the mouse and macaque brain in vivo. Resulting synthetic astrocytes and neurons (derived from cell-specific metabolite diffusion) can be compared with histological data. Very good agreement between Sholl analysis on real and synthetic astrocytes validates our approach and assumptions. We also measure increased size and complexity of synthetic astrocytes in primate compared to mouse, while dendritic organization appears better conserved throughout species. Although still in its infancy, our strategy opens new perspectives for the non-invasive evaluation of brain cell morphology.

In vivo 1H and 31P MR spectroscopy in healthy fibroglandular breast tissue at 7 Tesla. - Permission Withheld
Wybe JM van der Kemp1, Bertine L Stehouwer1, Vincent O Boer1, Peter R Luijten1, Dennis WJ Klomp1, and Jannie P Wijnen1
1Radiology, UMC Utrecht, Utrecht, Netherlands
Water and fat suppressed 1H total choline MR spectroscopy and 31P MR spectroscopy were performed in healthy fibroglandular breast tissue of a group of 8 volunteers. 31P T2 values were determined, and reproducibility of 1H and 31P MR spectroscopy were investigated. The 1H and 31P data were combined to calculate estimates of absolute concentrations of PC, GPC and PE.

Adjournment & Meet the Teachers

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