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Linescan BOLD and diffusion fMRI signal responses triggered by activation of the rat visual system differ in time, amplitude and shape
Denis Le Bihan1, Luisa Ciobanu1, Yukiko Masaki1,2, and Erwan Selingue1
1NeuroSpin, Gif-sur-Yvette, France, 2Shionogi & Co., Ltd., Osaka, Japan
High temporal resolution BOLD and diffusion fMRI using linescan show that the signal response time courses following visual stimulation in the rat visual system significantly differ in time, amplitude and shape, suggesting different mechanisms underlay the two approaches.

Figure 2: Example of results in a single subject

Top: BOLD fMRI showing Superior Colliculus and line position.

Middle: 2D/3D renderings of the space-time activation pattern (b=0) ([9 9] median filtering). The activation spot extends beyond the end of the stimulation.

Bottom: id with b=1800s/mm² showing 2 peaks with slightly different spatial extents, one inside the activation window and the other after the stimulation

Right: Response time courses. The response amplitude (beta) is higher for b=1800s/mm² and present 2 peaks. Both responses are statistically significant.

Figure 3: Group results

Left: Group average (n=6) for BP (15 points moving average). The amplitude increase with b value of the main peak and the shoulder are clearly visible.

Middle: Individual results (5 subjects) showing the differences in amplitude and in time-to-peak between b=0 and 1800s/mm² (p=0.016 and p=0.032, respectively).

Right: Group average (n=5) for ER (10 points moving average).