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Triple negative breast cancer COX-2 expression distinctly alters spleen metabolism in immunocompromised mice compared to immunocompetent mice

James Dion Barnett¹, Marie-France Penet¹, Balaji Krishnamachary ¹, Zaver Bhujwalla¹, Flonne Wildes¹, Santosh Kumar Bharti¹, and Yelena Mironchik¹
¹The Johns Hopkins University School of Medicine, Baltimore, MD, United States

Here we demonstrate how COX-2 expression may alter spleen metabolism that greatly impacts tumor progression and cancer persistence. Our findings suggest that tumor burden alone may drive metabolic alterations in the spleen.

Data summarizing the spleen weights in (A) SCID mouse groups with non-tumor-bearing control (n=6), SUM-149-EV (n=9), SUM-149-COX-2 (n=12) mice and (B) BALB/c mouse groups with non-tumor-bearing control (n=5), 4T1-wt (n=3), 4T1-EV (n=5) and 4T1-COX2shRNA (n=5) mice. Values represent Mean ± SEM.

Intensity quantification normalized to SCID mouse spleen weight from non-tumor-bearing control (n=6), SUM-149-EV (n=9), SUM-149-COX-2 (n=12) groups illustrated respectively for (A) glutamate, (B) glutamine, (C) glutathione, (D) lactate and (E) aspartate. Values represent Mean ± SEM.