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Functional MRI study of olfactory responses evoked by musk odor in the mouse whole brain under medetomidine anesthesia
Yumiko Tsubakihara1, Mitsuhiro Takeda1, Sosuke Yoshinaga1, and Hiroaki Terasawa1
1Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
Muscone is an odorant that attracts male mice. Muscone-evoked activations were identified in the mouse whole brain, using periodic stimulation and independent component analysis under medetomidine anesthesia.
Fig. 2 (A) Schematic illustration of the automated odor stimulation system. A drop of odorant solution is placed within a syringe pump, and the syringe becomes saturated with vapor. The saturated vapor is infused into the animals by operating the syringe pump and the electromagnetic valve. (B) Independent component analysis. Activations were detected as periodically occurring responses, even in the presence of large noise signals.
Fig. 3 (A) Number of muscone-evoked activations detected in the olfactory bulb and the olfactory cortex under different levels of medetomidine anesthesia. (B) Representative activation map detected at the 0.01 mg/kg/h medetomidine concentration, in the olfactory bulb, the piriform cortex, the olfactory tubercle, and the nucleus accumbens. In each slice, the distance from the bregma is shown at the bottom right of the panel.