Pierre-Marie Chevillard1, Martine Migaud1, and Nathalie Just1
1NhyRVana, INRAE, Nouzilly, France
1NhyRVana, INRAE, Nouzilly, France
Hypercapnic challenge measured with BOLD fMRI during long days versus short days was used to examine the potential of the BOLD response as an effective in-vivo marker of adult neurogensis in the sheep hypothalamus.
Group averaged T value maps comparing hypercania to baseline (One sample t-tests) at different time points during Long Days (LD) and during Short Days (SD). Arrows indicate statistically significant changes within the hypothalamus.
Figure 5. Immunohistochemistry data. A. Example of analysis in the arcuate nucleus (ARC) of the sheep hypothalamus near the 3rd ventricle showing the selection of a voxel of interest. Vessels (red surfaces) and stained neural stem cells (NSC) (SPOTS) can be seen. B. This analysis allowed statistical evaluation of the median distance between blood vessels (BV) and Sox2 stained NSCs demonstrating larger median distances during short day periods (SP) than long day periods (LP) in two different regions of the hypothalamus of sheep.